- 文本
- 历史
Abstract：Resveratrol, which is pres
Abstract：Resveratrol, which is present in grapes, wine and peanuts, is believed to possess chemoprotective properties such as anticarcinogenic effects and to provide protection against cardiovascular diseases. Little is known, however, about its intestinal absorption. We investigated the absorption and metabolism of resveratrol by using an isolated preparation of luminally and vascularly perfused rat small intestine. A synthetic perfusate free from blood components was used as vascular medium with a perfluorocarbon as oxygen carrier. Luminal media consisted of a bicarbonate buffered sodium chloride solution spiked with resveratrol in physiological, nutritionally relevant concentrations (28, 34 and 57 micromol/l, respectively). Viability was maintained during the entire perfusion and no significant differences between resveratrol and control perfusions for oxygen consumption, arterial pressure, lactate-pyruvate ratio and acid-base homeostasis were observed. Vascular uptake of luminally administered resveratrol was 20.5%. The majority of the absorbed resveratrol was conjugated to yield resveratrol glucuronide (16.8%), which was also the main luminal metabolite (11.2%). Lesser amounts of resveratrol sulfate, 3.0% and 0.3%, were found on the luminal and vascular side, respectively, while only minute amounts of resveratrol and resveratrol conjugates (1.9%) were found in the intestinal tissue. The structures of the resveratrol conjugates were verified by liquid chromatography coupled with mass spectometry (LC-MS). The results demonstrate an ample uptake and metabolic conversion of resveratrol. The proposed perfusion model serves as a tool to evaluate intestinal absorption and metabolic handling of phytochemicals, a pertinent input to the ongoing discussion about their health benefits.
0/5000
摘要：白藜芦醇，其存在于葡萄，酒和花生，被认为具有化学保护性能，如抗癌作用，并提供对抗心血管疾病的保护。然而鲜为人知的是，有关其肠道吸收。我们通过使用经腔内和血管灌注的大鼠小肠的分离的制备研究白藜芦醇的吸收和代谢。一种合成灌洗液免费从血液成分被用作具有全氟碳作为氧载体的血管平台。管腔培养基由白藜芦醇在生理，营养相关的浓度（28，34和57微摩尔/升，分别地）掺加碳酸氢缓冲氯化钠溶液。生存力在整个灌注过程中保持并观察耗氧量，动脉压，乳酸盐 - 丙酮酸比率和酸 - 碱平衡白藜芦醇和控制灌注之间没有显著差异。经腔内给予白藜芦醇的血管吸收为20.5％。大部分所吸收的白藜芦醇偶联，得到葡萄糖醛酸白藜芦醇（16.8％），这也是所述主腔的代谢物（11.2％）。较少量的硫酸白藜芦醇，3.0％和0.3％，分别在腔和血管侧，分别发现，而在肠组织中发现的白藜芦醇和白藜芦醇缀合物（1.9％）的仅微量的。白藜芦醇缀合物的结构通过加上质谱分析（LC-MS）液相色谱法证实。结果表明充足的摄取和白藜芦醇的代谢转化。所提出的灌注模型作为评价肠道吸收和植物化学物质的代谢处理，一个相关的输入到自己的健康益处正在讨论的工具。
正在翻译中..
Abstract：Resveratrol, which is present in grapes, wine and peanuts, is believed to possess chemoprotective properties such as anticarcinogenic effects and to provide protection against cardiovascular diseases. Little is known, however, about its intestinal absorption. We investigated the absorption and metabolism of resveratrol by using an isolated preparation of luminally and vascularly perfused rat small intestine. A synthetic perfusate free from blood components was used as vascular medium with a perfluorocarbon as oxygen carrier. Luminal media consisted of a bicarbonate buffered sodium chloride solution spiked with resveratrol in physiological, nutritionally relevant concentrations (28, 34 and 57 micromol/l, respectively). Viability was maintained during the entire perfusion and no significant differences between resveratrol and control perfusions for oxygen consumption, arterial pressure, lactate-pyruvate ratio and acid-base homeostasis were observed. Vascular uptake of luminally administered resveratrol was 20.5%. The majority of the absorbed resveratrol was conjugated to yield resveratrol glucuronide (16.8%), which was also the main luminal metabolite (11.2%). Lesser amounts of resveratrol sulfate, 3.0% and 0.3%, were found on the luminal and vascular side, respectively, while only minute amounts of resveratrol and resveratrol conjugates (1.9%) were found in the intestinal tissue. The structures of the resveratrol conjugates were verified by liquid chromatography coupled with mass spectometry (LC-MS). The results demonstrate an ample uptake and metabolic conversion of resveratrol. The proposed perfusion model serves as a tool to evaluate intestinal absorption and metabolic handling of phytochemicals, a pertinent input to the ongoing discussion about their health benefits.
正在翻译中..
摘要：白藜芦醇存在于葡萄、葡萄酒和花生中，具有抗癌、抗心血管疾病等化学保护作用。然而，对其肠道吸收的了解甚少。我们采用大鼠小肠腔内和血管灌注的分离制剂，研究白藜芦醇的吸收和代谢。以不含血液成分的合成灌流液为血管介质，全氟化碳为氧载体。管腔培养基包括生理、营养相关浓度（分别为28、34和57微摩尔/升）的碳酸氢钠缓冲氯化钠溶液和白藜芦醇。在整个灌流过程中，白藜芦醇和对照灌流在耗氧量、动脉压、乳酸丙酮酸比和酸碱平衡方面没有显著差异。腔内注射白藜芦醇的血管摄取率为20.5%。吸收的白藜芦醇大部分与白藜芦醇葡萄糖醛酸结合生成白藜芦醇葡萄糖醛酸（16.8%），白藜芦醇葡萄糖醛酸是白藜芦醇的主要代谢产物（11.2%）。管腔侧和血管侧的硫酸白藜芦醇含量分别为3.0%和0.3%，而肠组织中只有少量的白藜芦醇和白藜芦醇结合物（1.9%）。采用液相色谱-质谱联用技术（LC-MS）对白藜芦醇偶联物的结构进行了验证。结果表明，白藜芦醇具有丰富的吸收和代谢转化。所提出的灌注模型可作为评估肠道吸收和植物化学物质代谢处理的一个工具，对正在进行的有关其健康益处的讨论有一定的参考价值。
正在翻译中..
其它语言
本翻译工具支持: 世界语, 丹麦语, 乌克兰语, 乌兹别克语, 乌尔都语, 亚美尼亚语, 伊博语, 俄语, 保加利亚语, 信德语, 修纳语, 僧伽罗语, 克林贡语, 克罗地亚语, 冰岛语, 加利西亚语, 加泰罗尼亚语, 匈牙利语, 南非祖鲁语, 南非科萨语, 卡纳达语, 卢旺达语, 卢森堡语, 印地语, 印尼巽他语, 印尼爪哇语, 印尼语, 古吉拉特语, 吉尔吉斯语, 哈萨克语, 土库曼语, 土耳其语, 塔吉克语, 塞尔维亚语, 塞索托语, 夏威夷语, 奥利亚语, 威尔士语, 孟加拉语, 宿务语, 尼泊尔语, 巴斯克语, 布尔语(南非荷兰语), 希伯来语, 希腊语, 库尔德语, 弗里西语, 德语, 意大利语, 意第绪语, 拉丁语, 拉脱维亚语, 挪威语, 捷克语, 斯洛伐克语, 斯洛文尼亚语, 斯瓦希里语, 旁遮普语, 日语, 普什图语, 格鲁吉亚语, 毛利语, 法语, 波兰语, 波斯尼亚语, 波斯语, 泰卢固语, 泰米尔语, 泰语, 海地克里奥尔语, 爱尔兰语, 爱沙尼亚语, 瑞典语, 白俄罗斯语, 科西嘉语, 立陶宛语, 简体中文, 索马里语, 繁体中文, 约鲁巴语, 维吾尔语, 缅甸语, 罗马尼亚语, 老挝语, 自动识别, 芬兰语, 苏格兰盖尔语, 苗语, 英语, 荷兰语, 菲律宾语, 萨摩亚语, 葡萄牙语, 蒙古语, 西班牙语, 豪萨语, 越南语, 阿塞拜疆语, 阿姆哈拉语, 阿尔巴尼亚语, 阿拉伯语, 鞑靼语, 韩语, 马其顿语, 马尔加什语, 马拉地语, 马拉雅拉姆语, 马来语, 马耳他语, 高棉语, 齐切瓦语, 等语言的翻译.
