PVDF membrane was washed 4 times/15min with TBS-T, and incubated with horseradish peroxidase coupled anti-rabbit or anti-mouse Ig at room temperature for 45 minutes
PVDF membrane with TBS-T cleaning 4 times / 15min, with spicy root peroxidase coupled anti-rabbit or anti-rat Ig room temperature incubation for 45 minutes
PVDF membrane was washed with tbs-t 4 times / 15min and incubated with horseradish peroxidase coupled anti rabbit or anti mouse Ig for 45 minutes at room temperature<br>