On the basis of defining the concepts of big dataundefineds analysis ability, collaborative innovation ability and collaborative innovation performance and combing the relationship among them, this paper constructs the theoretical model of "big data analysis ability-collaborative innovation ability-cooperative innovation performance", and collects the relevant data of manufacturing enterprises through the professional data collection website-questionnaire star. SPSS22.0 and AMOS24.0 data analysis software are used to analyze the collected data, and the influence relationship among the variables is obtained, and the intermediary effect analysis is carried out. Through empirical research, this paper mainly draws the following conclusions: first, big dataundefineds tangible resources have a positive impact on big data human resources and big data intangible resources; second, inter-enterprise strategic coordination has a positive impact on organizational coordination and technological coordination; third, 3500 human resources and intangible resources have a positive impact on collaborative innovation ability; Fourth, the coordination of strategy, organization, technology and so on has a positive impact on the performance of collaborative innovation. Fifth, big data human resources and big data intangible resources play a complete intermediary role between big data tangible resources and collaborative innovation ability, and each dimension of collaborative innovation ability plays a complete intermediary role between the big data human resources, intangible resources and collaborative innovation performance. On the one hand, the research conclusion of this paper can enrich big dataundefineds analytical ability, collaborative innovation ability and other concepts, on the other hand, it can provide management enlightenment for enterprises to use big data to promote collaborative innovation among enterprises.