Sample Collection and RNA IsolationUmbilical cord blood from all infants in the total cohort was processed within 3 hours of delivery; 3 mL of cord blood was placed into Tempus Blood RNA tubes (AppliedBiosystems, Foster City, California) and biobanked at 80C. Total RNA was isolated from the Tempus system using the MagMAX for Stabilized Blood Tubes RNA Isolation Kit as per the manufacturer’s instructions (Ambion, Life Technologies, Austin, Texas). Isolated RNA was subsequently stored at 80C until further processing. Total RNA concentration was quantified using a NanoDrop 8000 Spectrophotometer (ThermoScientific NanoDrop,Wilmington, Delaware). In addition, RNA quality of all samples in the exploratory cohort were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies Inc, AgilentLaboratories, Santa Clara, California) to ensure purity of samples.
Sample Collection and RNA Isolation<br>Umbilical cord blood from all infants in the total cohort was processed within 3 hours of delivery; 3 mL of cord blood was placed into Tempus Blood RNA tubes (Applied<br>Biosystems, Foster City, California) and biobanked at 80C. Total RNA was isolated from the Tempus system using the MagMAX for Stabilized Blood Tubes RNA Isolation Kit as per the manufacturer’s instructions (Ambion, Life Technologies, Austin, Texas). Isolated RNA was subsequently stored at 80C until further processing. Total RNA concentration was quantified using a NanoDrop 8000 Spectrophotometer (ThermoScientific NanoDrop,<br>Wilmington, Delaware). In addition, RNA quality of all samples in the exploratory cohort were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies Inc, Agilent<br>Laboratories, Santa Clara, California) to ensure purity of samples.
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