对VEGFR-Fc融合蛋白SDS-聚丙烯酰胺凝胶电泳(简称SDS—PA

Validate the analysis method of VEGFR-Fc fusion protein SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (purity + molecular weight) to confirm that the analysis method can accurately detect the purity and molecular weight of the VEGFR-Fc fusion protein in the sample . Use SDS-polyacrylamide gel electrophoresis to verify the specificity, precision and durability. After SDS-polyacrylamide gel electrophoresis. The method verification results are: the specificity is the blank solution (auxiliary solution) without obvious interference; the light experiment sample, the detection aggregate has obvious changes; the precision is the repeatability of the non-reduced electrophoresis purity RSD of 0.12%, and the reduced electrophoresis purity RSD 0.62%, molecular weight RSD is 0.77%; intermediate precision: non-reduced electrophoresis purity RSD between different personnel is 0.62%, reduced electrophoresis purity RSD is 0.75%, molecular weight RSD is 1.21%; durability is the use of 3 different batches of prefabricated Gel non-reducing electrophoresis purity RSD is 0.78%, reducing electrophoresis purity RSD is 0.42%, and molecular weight RSD is 0.81%. According to the above verification results, it is proved that the SDS-polyacrylamide gel electrophoresis (test stain) method meets the requirements in terms of specificity, precision and durability. Therefore, this method is suitable for the purity and molecular weight of the VEGFR-Fc fusion protein in the sample. Detection.

The analysis method of VEGFR-Fc fusion protein SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (purity plus molecular weight) was verified to confirm that the analysis method can accurately detect the purity and molecular weight of VEGFR-Fc fusion protein in the sample. Electrophoresis with SDS-polyacrylamide gel is verified in specific properties, precision and durability. Electrophoresis through SDS-polyacrylamide gel. The methodological verification results are: no obvious interference in the detection of blank solution (auxiliary solution), no obvious change in the detection of the polymer in the lighting experimental sample, 0.12% RSD for non-reductive electrophoresis purity and 0.0.RSD for reduced electrophoresis purity. 62%, molecular weight RSD 0.77%, intermediate precision: non-reductive electrophoresis purity RSD 0.62%, reduced electrophoresis purity RSD 0.75%, molecular weight RSD 1.21% Durability is 0.78% for non-reductive electrophoresis purity RSD using 3 different batches, 0.42% for reduced electrophoresis purity and 0.81% molecular weight RSD. Based on the above verification results, it is proved that the SDS-polyacrylamide gel electrophoresis (testing) method meets the requirements in terms of specific properties, precision and durability, so it is suitable for the testing of VEGFR-Fc fusion protein purity and molecular weight in the sample.

The VEGFR-Fc fusion protein SDS- polyacrylamide gel electrophoresis (SDS PAGE) (purity + molecular weight) analysis method was validated to confirm that the method can accurately detect the purity and molecular weight of VEGFR-Fc fusion protein in the sample. The specificity, precision and durability were verified by SDS- polyacrylamide gel electrophoresis. SDS- polyacrylamide gel electrophoresis. The results of methodology validation were as follows: specificity: blank solution (auxiliary solution) detection had no obvious interference; light test sample, detection of polymer had obvious changes; precision: RSD of non reduction electrophoresis purity was 0.12%, RSD of reduction electrophoresis purity was 0.62%, RSD of molecular weight was 0.77%; intermediate precision: RSD of non reduction electrophoresis purity was 0.62%, RSD of reduction electrophoresis purity was 0.75% The relative standard deviation (RSD) of molecular weight was 1.21%, the RSD of non reduction electrophoresis was 0.78%, the RSD of reducing electrophoresis was 0.42%, and the RSD of molecular weight was 0.81%. According to the above verification results, the SDS- polyacrylamide gel electrophoresis method is suitable for specificity, precision and durability. Therefore, the method is suitable for the detection of the purity and molecular weight of VEGFR-Fc fusion protein in samples.<br>