Diluent and Borate solution: Procee

Diluent and Borate solution: Proceed as directed in the AssaySoltion C: 4 mg/mL of9-fluorenylmethyl chloroformate in acetonitrile. [Note-Prepare this solutionfresh just before use.JStandard stock solution: 0.6 mg/mL of USP Alendronate Sodium RS in DiluentStandard solution A: Transfer 5.0 mL of the Standard stock solution to a 50-mL polypropylene, screwcap centrifuge tube containing 5 mL of Borate solution. Add 5 mL of acetonitrile and 5 mL ofSolutionC, and shake for 45 s. Allow to stand at room temperature for 30 min. Add 20 mL of methylenechloride, and shake vigorously for 1 min Centrifuge for 5-10 min, and use a portion of the clear upperaqueous layerStandard solution B: 0.6 ug/mL ofUSP Alendronate Sodium RS in Diluent from Standard stock solution.Transfer 5 mL of this diluted solution(0.6 ug/mL) to a 50-mL polypropylene, screw-cap centrifugetube containing 5 mL of Borate solution. Add 5 mL of acetonitrile and 5 mL of Solution C, and shakefor 45s. Allow to stand at room temperature for 30 min. Add 20 mL of methylene chloride, and shakevigorously for 1 min. Centrifuge for 5-10 m5-10 min, and use a portion of the clear upper aqueous layer.Reagent blank: Transfer 5.0 mL of Diluent to a 50-mL polypropylene, screw-cap centrifuge tubecontaining 5 mL of Borate solution. Add 5 mL of acetonitrile and 5 mL of Solution C, and shake for 45s. Allow to stand at room temperature for 30 min. Add 20 mL of methylene chloride, and shakevigorously for I min Centrifuge for 5-10 in, and use a portion of the clear upper aqueous layerSample stock solution: 0.6 mg mL of Alendronate Sodium in DiluentSample solution: Transfer 5.0 mL of Sample stock solution to a 50-mL polypropylene, screw-capcentrifuge tube containing 5 mL of Borate solution. Add 5 mL of acetonitrile and 5 mL of Solution Cand shake for 45 s. Allow to stand at room temperature for 30 min. Add 20 mL of methylene chlorideand shake vigorously for I min Centrifuge for 5-10 min, and use a portion of the clear upper aqueouslaverChromatographic system