Biological imaging experiments requ

Biological imaging experiments require sufficient signal-tonoiseratio to reveal minute specimen details, allow robustimage analysis and eventually lead to conclusive and reliableexperimental results. The issue of signal and noise is especiallyimportant in case of dim and sensitive fluorescent specimens,where insight is often limited by the image noise rather thanby the microscope’s resolution. There are a few well-knownmethods to increase the signal-to-noise ratio, such as prolongingthe camera exposure time or increasing the illuminationintensity. Unfortunately, these methods also lead to slowerexperiments, and to increased specimen damage, photobleachingand phototoxicity. However, there is also a lessknown method that has recently become attractive due to theadvances in the high-resolution microscope cameras, specificallythe increasing sensor sizes and pixel counts. This method2ZEISS Axiocam FamilyCollect More Photons By Choosing theRight Camera Adapter for Your Application.Authors: Uros Krzic, Horst Wolff, Markus CappellaroCarl Zeiss Microscopy GmbHDate: November 2019Figure 1 Effect of the SNR on an image. left Fluorescence images of mitochondria acquired at four different imaging conditions with decreasing SNR.Comparedto the first image, subsequent images have 2×, 4× and 8× lower SNR. right Magnified region of the images on the left indicated by the yellow rectangle.While all four images were recorded with identical microscope resolution, low SNR in the nosier images severely limits the ability to resolve small details.uses demagnifying camera adapters to match the field of viewof the microscope with the size of the camera’s image sensor.Demagnifying adapter allows the sensor to capture more ofthe light collected by the microscope’s objective lens and thusincrease the sensitivity of your microscope system. Additionally,the adapter enlarges the microscope’s field of view and enablesthe user to capture a larger part of the specimen witha single image. Consequently, the adapter also dramaticallyaccelerates tiling experiments