nstructions for useMix your buffers freshly using high-purity components and ultrahigh purity water such as Milli-Q or Nanopure. Filter buffers through a 0.2 or 0.45 μm filter and degas before use. This will remove particulates and help reduce the risk of bacterial growth, which could otherwise damage the column and your UHPLC or HPLC system.– It is important to ensure that your sample is fully dissolved and does not precipitate in the high salt concentration mobile phase. Protein concentrations of around 1 mg/mL will give excellent results.For best peak shape, prepare samples in conditions as close to the initial mobile phase conditions as possible, including matching pH