血管生成能够促进组织修复和再生（包括植入物体骨整合），因此如何提高植入

Angiogenesis can promote tissue repair and regeneration (including osseointegration of implanted objects). Therefore, how to improve the vascularization ability of endothelial cells around implants has attracted widespread attention. As we all know, macrophages are important immune cells in organisms, and it has been confirmed that they play an immunomodulatory function in angiogenesis. Although studies have shown that exosomes derived from macrophages are involved in this process, whether changes in the cellular microenvironment cause changes in exosomes secreted by macrophages is still unknown. Cobalt is usually used as an implant additive to simulate the hypoxia microenvironment. The latter can stabilize the hypoxia-inducible factor-1α of macrophages and endothelial cells, and promote the expression of endothelial growth factor to induce angiogenesis. In this paper, different concentrations of cobalt ions (0-200 μM) on the activity, proliferation, morphology, skeleton, adhesion, and phenotypic gene expression of macrophages were studied, and on this basis, different concentrations of cobalt ions (0-200 μM) were used to extract different The concentration of cobalt ion stimulates the exosomes secreted by macrophages; the macrophage-derived exosomes were identified by scanning electron microscopy, particle size analysis and Western Blot; and each group of macrophage-derived exosomes was evaluated. The effect of endothelial cell activity, proliferation, morphology, skeleton, adhesion, migration, in vivo and in vitro angiogenesis, NO expression, VEGF secretion, angiogenesis-related gene expression and integrin transport. The specific results are as follows:

Angiogenesy promotes tissue repair and regeneration, including bone integration in implanted objects, so there is widespread concern about how to improve the ability of endoskin cells around implants to form blood vessels. It is well known that macrophages, as important immune cells of organisms, have been shown to play an immunomodulation function in angiogenesics. Although studies have shown that exosomes from macrophage sources are involved in the process, it is not known whether changes in the micro-environment of cells cause changes in exosomes secreted by macrophages. Cobalt is commonly used as an implant additive to simulate the micro-environment of hypoxia, which stabilizes the hypoxia-1 alpha of macrophages and endodertic cells, and promotes the expression of endoderging factors to induce angiogenesy. In this paper, the expression of macrophage activity, proliferation, profiling, profiling, skeleton, adhesion and esoteric genes of different concentrations of cobalt ions (0-200 μM) is studied, and different concentrations of cobalt ion concentrations are extracted to stimulate macrophage secretion. exosomes, identification of macrophage-based exosomes by scanning electroscopes, particle size analysis and Western Blot, and evaluation of the activity, proliferation, profiling, shape, skeleton, adhesion, migration, Effects of in vivo angiovascular, NO expression, VEGF secretion, expression of vascular-related genes, and transport of allicins. The results are as follows:

Angiogenesis can promote tissue repair and regeneration (including osseointegration), so how to improve the angiogenesis ability of endothelial cells around the implant has been widely concerned. As we all know, macrophages, as an important immune cell, have been proved to play an immunomodulatory role in angiogenesis. Although some studies have shown that macrophage derived exosomes are involved in this process, it is not known whether the change of cell microenvironment causes the change of exosomes secreted by macrophages. Cobalt is usually used as an implant additive to simulate hypoxic microenvironment, which can stabilize hypoxia inducible factor-1 α in macrophages and endothelial cells, and promote the expression of endothelial growth factor to induce angiogenesis. The activity, proliferation, morphology, skeleton, adhesion and phenotypic gene expression of macrophages stimulated by different concentrations of cobalt ion (0-200 μ m) were studied. On this basis, the exosomes secreted by macrophages stimulated by different concentrations of cobalt ion were extracted by differential centrifugation. The results were analyzed by scanning electron microscope, particle size analysis and Western blot The activity, proliferation, morphology, skeleton, adhesion, migration, angiogenesis in vivo and in vitro, no expression, VEGF secretion, expression of angiogenesis related genes and integrin transport of endothelial cells were evaluated. The results are as follows<br>