由于[(PSS/PPy)(P2Mo18/PPy)n]多层复合膜修饰电极

Since [(PSS / PPy) (P2Mo18 / PPy) n] Good multilayer composite membrane modified electrode catalytic effect on tyrosinase, and a modified cyclic voltammogram of the electrode of the first reduction peak in the vicinity of -0.25 V, thus at a constant potential of -0.25 V, was added to the reaction system 4mL1: 9 (0.1mL • L-1 of sulfuric acid + 0.5mL • L-1 sodium sulfate) mixed solution as a supporting electrolyte, a total scan time of 600 seconds per scan 30 seconds Add 0.4mL 40.305U • mL-1 tyrosinase, multipoint magnetic stirrer on rapid mixing of the catalytic current monitoring response timing polyoxometalates. According to the chronoamperometry then tyrosinase concentration plotted with respect to a linear standard curve can be obtained can be calculated detection limit of the modified electrode. Can be seen from Figure 9, each time with the addition of tyrosinase solution, the electrode response current will increase appears stepwise, i.e., with an increase in catalytic current increases the concentration of tyrosinase, then the current timing with respect to a standard curve of tyrosinase concentration (FIG. 10), in the 3.66U • mL-1 ~ 26.87U • mL-1 linear curve within the range of I = 0.16108c-16.35976, R2 = 0.9969, the lowest detection limit (S / N = 3) is 2.67473U • mL-1

Since the multi-layer composite membrane modification electrode has good catalytic effect on tyrosine, and the first reduction peak in the circulating volta map of the modified electrode is near -0.25 V, therefore, at a constant potential -0.25 V, in the reaction system Add 4mL1:9 (0.1mL-L-L-1 sulfate sodium sulfate and 0.5mL-L-1 sodium sulfate) as a supportelectrolyte, with a total scan time of 600 seconds, 0.4mL 40.305U.mL-1 tyrosine per scan for 30 seconds, The timing response of the catalytic current to polyoxometalates is monitored rapidly on a multi-point magnetic agitator. Then according to the timed current relative to the tyrosine concentration graph can obtain a linear standard curve, the modified electrode detection limit can be calculated. As can be seen from Figure 9, with the addition of each tyrosine solution, the response current of the electrode will then increase with the step, i.e. the catalytic current increases with the concentration of tyrosine enzyme, and then the standard curve of the timing current relative to the concentration of tyrosine enzyme (shown in Figure 10) can be seen, in 3.66U-mL-1 The linear curve in the range of 26.87U-mL-1 is I-0.16108c-16.35976, R2-0.9969, and the minimum detection limit (S/N-3) is 2.67473U-mL-1

Because [(PSS / PPy) (p2mo18 / PPy) n] multilayer composite film modified electrode has a good catalytic effect on tyrosinase, and the first reduction peak in the cyclic voltammogram of the modified electrode is near - 0.25 V, so it is at a constant potential of - 0.25 v Then, 4ml 1:9 (0.1ml · L-1 sulfuric acid + 0.5ml · L-1 sodium sulfate) mixed solution was added into the reaction system as the supporting electrolyte. The total scanning time was 600 seconds, and 0.4ml 40.305u · ml-1 tyrosinase was added every 30 seconds. The timing response of the catalytic current to polyoxometalates was quickly monitored on the multi-point magnetic stirrer. Then according to the time current relative to the concentration of tyrosinase, the linear standard curve can be drawn, and the detection limit of the modified electrode can be calculated. It can be seen from Figure 9 that with the addition of each tyrosinase solution, the response current of the electrode will increase step by step, that is, the catalytic current will increase with the increase of the concentration of tyrosinase. From the standard curve of the timing current relative to the concentration of tyrosinase (as shown in Figure 10), it can be seen that at 3.66u • ml-1 ~The linear curve is I = 0.16108c-16.35976, R2 = 0.9969, and the minimum detection limit (s / N = 3) is 2.67473u • ml-1