R0 Parameter After application of base (control), R0 values tended to decrease throughout the study period of 8 weeks, while R0 values after application of active formulation tended to decrease slightly, but no pronounced effects were seen (Fig. 2).The ANOVA revealed that both base and active formulation have insignificant status for R0 values. When the paired t-test was applied to reveal any correlation between the two formulations, a positive relation was found in the weeks 1, 4 and 6. In this study, R0 values by formulation showed that it had no effect on R0 parameters of skin mechanical properties.