50只SPF级雄性昆明小鼠，随机选取10只小鼠用于检测造模是否成功。剩

50只SPF级雄性昆明小鼠，随机选取10只小鼠用于检测造模是否成功。剩余40只小鼠每组8只，分别为正常对照组、模型对照组、化疗前使用地榆升白片组（以下简称化疗前给药组）、化疗中使用地榆升白片组（以下简称化疗中给药组）、化疗后使用地榆升白片组（以下简称化疗后给药组）。各组小鼠灌胃14天，正常对照组腹腔注射0.9％氯化钠注射液（100mg/kg/d，NS），其余组小鼠均腹腔注射环磷酰胺（100mg/kg/d，CTX），连续3天。正常对照组，第1天至第14天（d1-d14）予NS灌胃，d3-d5予NS腹腔注射；模型对照组，d1-d14予NS灌胃，d3-d5予CTX腹腔注射；化疗前给药组，d1-d14予地榆升白片溶液灌胃，d3-d5予CTX腹腔注射；化疗中给药组，d1-d2予NS灌胃，d3-d5予CTX腹腔注射，d3-d14予地榆升白片溶液灌胃；化疗后给药组，d1-d6予NS灌胃，d3-d5予CTX腹腔注射，d7-d14予地榆升白片溶液灌胃。造模结束后，将用于检测造模的小鼠拔出眼球取血，检测外周血白细胞、红细胞、血红蛋白、血小板以验证造模是否成功。其余各组小鼠于第6天和第15天（最后一次灌胃24小时后），断尾取血法检测外周血白细胞变化情况；拔出小鼠眼球取血，用酶联免疫吸附测定（ELISA）法检测离心后获得的小鼠血清GM-CSF表达水平；取出双侧股骨骨髓制成涂片，用免疫组化（SP）法检测骨髓细胞Bax及Bcl-2表达水平。实验期间详细记录各实验组小鼠的一般情况。

50 SPF grade male Kunming mice were randomly selected and 10 mice were used to test the success of modeling. The remaining 40 mice were divided into a normal control group, a model control group, a group treated with Diyu Shengbai Tablets before chemotherapy (hereinafter referred to as the pre chemotherapy administration group), a group treated with Diyu Shengbai Tablets during chemotherapy (hereinafter referred to as the pre chemotherapy administration group), and a group treated with Diyu Shengbai Tablets after chemotherapy (hereinafter referred to as the post chemotherapy administration group). Mice in each group were gavaged for 14 days, while the normal control group was intraperitoneally injected with 0.9% sodium chloride injection (100mg/kg/d, NS). The other groups of mice were intraperitoneally injected with cyclophosphamide (100mg/kg/d, CTX) for 3 consecutive days. The normal control group was given NS by gavage from day 1 to day 14 (d1-d14), and NS was injected intraperitoneally from day 3 to day 5; In the model control group, NS was administered orally on d1-d14 and CTX was intraperitoneally injected on d3-d5; Before chemotherapy, the medication group was given Diyu Shengbai Tablet solution by gavage on d1 to d14, and CTX intraperitoneal injection on d3 to d5; In the chemotherapy group, NS was administered by gavage on d1-d2, CTX was administered intraperitoneally on d3-d5, and Diyu Shengbai Tablet solution was administered by gavage on d3-d14; After chemotherapy, the medication group was given NS by gavage on d1 to d6, CTX by intraperitoneal injection on d3 to d5, and Diyu Shengbai Tablet solution by gavage on d7 to d14. After the modeling is completed, the eyeballs of the mice used for testing the modeling will be extracted for blood collection, and peripheral blood white blood cells, red blood cells, hemoglobin, and platelets will be tested to verify the success of the modeling. On the 6th and 15th days (24 hours after the last gavage) of the remaining groups of mice, the changes in peripheral blood white blood cells were detected by tail cutting blood collection method; Extract the mouse eyeballs and take blood, and use enzyme-linked immunosorbent assay (ELISA) to detect the expression level of GM-CSF in the mouse serum obtained after centrifugation; Take out bilateral femoral bone marrow and make a smear, and use immunohistochemical (SP) method to detect the expression levels of Bax and Bcl-2 in bone marrow cells. Record in detail the general situation of each experimental group of mice during the experiment.

50 SPF male Kunming mice, 10 mice were randomly selected to test whether the modeling was successful. The remaining 40 mice were divided into normal control group, model control group, Sanguisorba Shengbai tablet group before chemotherapy (hereinafter referred to as the administration group before chemotherapy), Sanguisorba Shengbai tablet group during chemotherapy (hereinafter referred to as the administration group during chemotherapy) and Sanguisorba Shengbai tablet group after chemotherapy (hereinafter referred to as the administration group after chemotherapy). Mice in each group were gavaged for 14 days. The normal control group was intraperitoneally injected with 0.9% sodium chloride injection (100mg/kg/d, NS), and the other groups were intraperitoneally injected with cyclophosphamide (100mg/kg/d, CTX) for 3 consecutive days. In the normal control group, NS was given intragastrically from day 1 to day 14 (d1-d14) and NS was given intraperitoneally from D3 to D5. Model control group, d1-d14 was given NS intragastrically, d3-d5 was given CTX intraperitoneally; In the administration group before chemotherapy, d1-d14 were given Sanguisorba Shengbai tablet solution by gavage, d3-d5 were given CTX intraperitoneal injection; In the chemotherapy group, NS was given by gavage on d1-d2, CTX was given by intraperitoneal injection on d3-d5, and Diyu Shengbai Tablet solution was given by gavage on d3-d14. In the administration group after chemotherapy, NS was given by gavage at d1-d6, CTX was given by intraperitoneal injection at d3-d5, and Diyu Shengbai Tablet solution was given by gavage at d7-d14. After the modeling, the mice used to detect the modeling were pulled out of their eyeballs to take blood, and the peripheral white blood cells, red blood cells, hemoglobin and platelets were detected to verify whether the modeling was successful. On the 6th and 15th day (24 hours after the last gastric perfusion), the changes of peripheral blood leukocytes were detected by tail cutting and blood sampling. The eyeball of mice was pulled out to take blood, and the expression level of GM-CSF in serum of mice obtained after centrifugation was detected by enzyme-linked immunosorbent assay (ELISA). Bilateral femoral bone marrow was taken out to make smears, and the expression levels of Bax and Bcl-2 in bone marrow cells were detected by immunohistochemistry (SP). The general situation of mice in each experimental group was recorded in detail during the experiment.