Seismic fragility curves, which defines the probability of reaching or exceeding a specified damage state given different ground motion intensity measures, are very powerful tools for seismic vulnerability assessment. Soil-pile interaction (SPI) has been found to have a significant impact on seismic performance of pile-supported wharf structures(PSWSs). SPI is a complex process involving inertial and kinematic interaction between piles and soil, time-varying pore-water pressure, and soil nonlinearity. In this study, the focus is placed on seismic fragility evaluation of the PSWS as well as on the SPI effect. Specifically, the seismic fragility of a large-scale PSWS located at the Port of Los Angeles Berth 100 is fully investigated with and without considering SPI. Inaddition, to accurately classify the different damage states for fragility assessment, the pushover analysis strategy is utilized to determine the bound limits of seismic demands of this large-scale PSWS.