Sequential labeling of formaldehyde-fixed HeLa cells with two Tyramide Amplification Kits. Mitochondria (green) visualized with rabbit anti-COX IV primary antibody and Tyramide Amplification Kit with HRP goat anti-rabbit IgG and CF®488A-tyramide, followed by peroxidasequenching. Nucleoli (magenta) visualized with mouse anti-cyclin B1 primary antibody and Tyramide Amplification Kit with HRP goat anti- mouse IgG and CF®568-tyramide. Actin (red) detected with CF®640R-phalloidin, and cell nuclei (blue) stained with DAPI