此外，基于这种聚合酶/切口内切核酸酶 DNA 机器和多个引物样 RCA

Furthermore, based on this polymerase/nicking endonuclease DNA machine and multiple primer-like RCAs, we designed a one-step rapid fluorescent biosensing method for ultrasensitive detection of the BCR-ABL1 fusion gene (Fig. 5D). In the strategy, the BCR-ABL1 fusion gene can be specifically identified by using dual probes to form a three-way junction structure, and then a large number of triggers are generated by the SDA reaction, which initiates the downstream nick-mediated RCA reaction. Introducing two nick endonuclease recognition sites in a circular DNA template, allowing RCA to occur in a multiple primer-like manner, the developed method yielded a broad linear response from 10fM to 1nM with a low detection limit of 5.52 fM.

In addition, based on this polymerase/incision endonuclease DNA machine and multiple primer like RCAs, we designed a one-step rapid fluorescent biosensor method for ultra sensitive detection of BCR-ABL1 fusion gene (Figure 5D). In the strategy, the BCR-ABL1 fusion gene can be specifically identified by using a triple junction structure formed by dual probes, and then a large number of triggering factors can be generated through SDA reaction, which initiates downstream incision mediated RCA response. Two nick endonuclease recognition sites were introduced into the circular DNA template to make RCA occur in a multiple primer like manner. The developed method produced a wide linear response from 10fM to 1nM, with a low detection limit of 5.52 fM.

In addition, based on this polymerase/endonuclease DNA machine and multiple primer-like RCA, we designed a one-step rapid fluorescence biosensor for ultra-sensitive detection of BCR-ABL1 fusion gene (Figure 5D). In this strategy, BCR-ABL1 fusion gene can be specifically identified by using double probes to form a three-way junction structure, and then a large number of trigger factors can be generated by SDA reaction, which starts the downstream incision-mediated RCA reaction. Two notch endonuclease recognition sites were introduced into the circular DNA template, so that RCA occurred in a multi-primer-like manner. The developed method produced a wide linear response from 10fM to 1nM, with a low detection limit of 5.52 fM.