Statistical Analysis. Analysis of v

Statistical Analysis. Analysis of variance (ANOVA) was performed using general linear model (GLM) in Minitab 16 (Minitab Inc., State College, PA, USA) to determine the statistical effects of design variables on the yield parameters, PNC, GLS, and sensory quality parameters. Analysis of variance was also conducted for each location and year for the different treatments. GLM analysis was performed using fertilizer treatment, location, and year as main factors, whereas interactions between main factors and replicates were nested within year and location. For the sensory analyses, the individual assessor was considered as random (main) factor, whereas the other factors were fixed. Year and session in sensory analysis were confounded. Tukey’s test was used to confirm effect of individual fertilizer treatments.Loading plot and score plots from PCA of broccoli grown with different fertilizer materials at a southern (Grimstad) and a northern location (Bodø) for two years (2009 and 2010). The first two principal components explain 52.0% of the variation in GLS content, N and phenological parameters, and sensory attributes. Fertilizer ID abbreviations: NF, no fertilizer; MF, mineral fertilizer; SM, sheep manure; SS, shrimp shell; GLS, total glucosinolates; ALI, total aliphatic; IND, total indolic; GLI, glucoiberin; GLR, glucoraphanin; 4-OHGLB, 4-hydroxy-lucobrassicin; GLB, glucobrassicin; 4MGLB, 4-methoxyglucobrassicin; NGLB, neoglucobrassicin.