P-STAT3 is a marker of activation of IL-6 signaling pathway [33,34]. TNF-α is an important pro-inflammatory factor, which can induce inflammatory cascade reaction and mediate apoptosis of liver cells and promote serious liver injury [35,36]. The results showed that when the expression of Gp96 was inhibited, the expression of TNF-α was significantly up-regulated, while the expression of p-STAT3 was significantly down-regulated in the liver of Gp96-sgRNA3+alcohol mice compared with the saline+alcohol mice (P