电离辐射对人类健康和公共安全构成潜在的风险。因此，急需一款在辐射暴露早

Ionizing radiation poses a potential risk to human health and public safety. Therefore, an urgent need in the early stages of exposure to radiation can quickly minimally invasive biomarkers convenient testing to assess the extent of human suffering ionizing radiation. Serum protein is a promising biomarker, it has found that some radiation-sensitive protein, but for the low dose and high linear energy transfer (LET) radiation of the protein has not been reported. In our study, the mouse systemic exposure of carbon ions at 0.05 ~ 1gy or x-rays of 0.5 ~ 4gy. Then, we use the expression profile of mouse antibodies after exposure to radiation detection array 6 to 24 hours to serum proteins, and enzyme-linked immunosorbent assay further verified. We found that pancreatic insulin-like growth factor binding protein -3 (IGFBP-3) for the difference in time and dose-dependent expression of the presence of ionizing radiation. Further, receiving - operating characteristics analysis showed that serum IGFBP-3 with high sensitivity and high specificity is effective in exposed individuals. The combination of these results, we believe that high serum IGFBP-3, is extremely sensitive to low let ionizing radiation can be used as indicators of a new minimally invasive radiation exposure.

电离辐射对人类健康和公共安全构成潜在的风险。因此，急需一款在辐射暴露早期阶段就能快速便捷检测的微创生物标记物，用来评估人体遭受电离辐射的程度。血清蛋白是一种很有希望的生物标志物，目前已发现一些放射敏感蛋白，但针对低剂量和高线性能量转移(LET)辐射的蛋白尚未见报道。在我们的研究中，小鼠全身暴露在0.05 ~ 1gy的碳离子或0.5 ~ 4gy的x射线下。随后，我们使用小鼠抗体阵列检测辐射暴露后6 ~ 24小时血清蛋白的表达谱，同时，采用酶联免疫吸附法进一步验证。我们发现胰胰岛素样生长因子结合蛋白-3 （IGFBP-3）对电离辐射存在时间和剂量依赖的表达差异。此外，接受-操作特征分析显示，血清IGFBP-3对高敏感性和高特异性的暴露个体是有效的。综合这些结果，我们认为血清IGFBP-3对高、低let电离辐射极为敏感，可作为一种新的辐射暴露的微创指标。

Ionizing radiation poses a potential risk to human health and public safety. Therefore, there is an urgent need for a minimally invasive biomarker that can be quickly and easily detected in the early stage of radiation exposure to assess the degree of human exposure to ionizing radiation. Serum protein is a promising biomarker, and some radiosensitive proteins have been found. However, there is no report on the low dose and high linear energy transfer (let) radiation proteins. In our study, mice were exposed to 0.05-1gy of carbon ions or 0.5-4gy of X-rays. Subsequently, we used mouse antibody array to detect the expression profile of serum protein 6-24 hours after radiation exposure, and further verified it by enzyme-linked immunosorbent assay. We found that insulin-like growth factor binding protein-3 (IGFBP-3) had time-dependent and dose-dependent expression differences on ionizing radiation. In addition, the analysis of receptive operational characteristics showed that serum IGFBP-3 was effective for individuals with high sensitivity and specificity. Based on these results, we believe that serum IGFBP-3 is very sensitive to high and low let ionizing radiation and can be used as a new minimally invasive indicator of radiation exposure.<br>