电离辐射对人体健康和公共安全构成重大威胁。由于目前指示辐射暴露的方法存

Ionizing radiation on human health and pose a major threat to public safety. Due to the limitations inherent in the current method of indicating exposure to radiation, and therefore needs to be easily and quickly detect a new minimally invasive biomarkers in the early stages, for the best medical treatment. Serum protein is an attractive biomarker has been found that some radiation-sensitive protein, but for the low dose and high linear energy transfer (LET) radiation of the protein has not been reported. In this study, mice systemic exposure of carbon ions at different doses and x-ray. We used mouse antibody arrays detect protein expression profiles of serum after 24 hours. After screening conditions using ELISA further verified IGF -1 (IGF-1), insulin-like growth factor binding protein -1 (IGFBP-1), IGFBP-3. After exposure to carbon ions and 0.05 ~ 1gy 0.5 ~ 4gy x-ray, and only with the dose of IGFBP-3 showed a significant increase increases, both the x-ray or carbon ions. Detecting IGFBP-3, to observe the changes over time. The results show that 6 to 24 hours after the carbon ions and x-ray irradiation, the expression of IGFBP-3 discrepancies. Further, receiving - operating characteristics analysis showed that serum IGFBP-3 with high sensitivity and high specificity is effective in exposed individuals. These results suggest that high serum IGFBP-3, is extremely sensitive to ionizing radiation let a low, minimally invasive and early indicator reaction, as a new radiation exposure.

电离辐射对人体健康和公共安全构成重大威胁。由于目前指示辐射暴露的方法存在固有的局限性，因此需要在早期阶段容易和快速检测出新的微创生物标记物，以便进行最佳的医疗治疗。血清蛋白是有吸引力的生物标志物，目前已发现一些放射敏感蛋白，但针对低剂量和高线性能量转移(LET)辐射的蛋白尚未见报道。在这项研究中，小鼠全身暴露在不同剂量的碳离子和x射线下。我们使用小鼠抗体阵列检测24小时后血清蛋白的表达谱。条件筛选后，采用酶联免疫吸附法进一步验证胰岛素样生长因子-1 (IGF-1)、胰岛素样生长因子结合蛋白-1 (IGFBP-1)、IGFBP-3。在暴露于0.05 ~ 1gy的碳离子和0.5 ~ 4gy的x射线后，只有IGFBP-3随剂量的增加表现出明显的增加，无论是碳离子还是x射线。检测IGFBP-3，观察其随时间的变化。结果表明，在碳离子和x射线照射后6 ~ 24小时，IGFBP-3的表达出现差异。此外，接受-操作特征分析显示，血清IGFBP-3对高敏感性和高特异性的暴露个体是有效的。这些结果提示，血清IGFBP-3对高、低let电离辐射极为敏感，并可早期反应，可作为一种新的辐射暴露的微创指标。

Ionizing radiation poses a major threat to human health and public safety. Due to the inherent limitations of current methods of indicating radiation exposure, it is necessary to detect new minimally invasive biomarkers easily and quickly in the early stage in order to carry out the best medical treatment. Serum proteins are attractive biomarkers, and some radiosensitive proteins have been found. However, there is no report on the proteins targeting low dose and high linear energy transfer (let) radiation. In this study, mice were exposed to different doses of carbon ions and X-rays. We used mouse antibody array to detect the expression profile of serum protein after 24 hours. After the selection of conditions, IGF-1, IGFBP-1 and IGFBP-3 were further verified by enzyme-linked immunosorbent assay. After exposure to 0.05-1gy of carbon ions and 0.5-4gy of X-rays, only IGFBP-3 showed a significant increase with the increase of dose, both carbon ions and X-rays. IGFBP-3 was detected and its change with time was observed. The results showed that the expression of IGFBP-3 was different between 6 and 24 hours after carbon ion and X-ray irradiation. In addition, the analysis of receptive operational characteristics showed that serum IGFBP-3 was effective for individuals with high sensitivity and specificity. These results suggest that serum IGFBP-3 is very sensitive to high and low let ionizing radiation, and can react early, and can be used as a new minimally invasive indicator of radiation exposure.