十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)是用于检测蛋白质

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a method for detecting the effect of protein separation. This method is based on the difference in molecular weight of protein subunits to achieve protein separation. In this experiment, recombinant human follicle stimulating hormone (rhFSH) was used as a sample, and the separation of the gel and protein bands were studied by SDS-PAGE electrophoresis. The gel separation effect of different SDS content is different, and the protein bands detected by different loading volumes are also different. In this experiment, the separation effect of 8% and 13.5% gels was first compared, and it was concluded that the 13.5% gel was suitable for the detection of rhFSH protein. Secondly, change the sample loading volume, use the selected 13.5% gel for electrophoresis detection, and compare and analyze the gel chart to find that different loading volumes have different effects on the gel band. The suitable loading volume for rhFSH samples is 10 μL. At the same time, the protein activity of rhFSH was studied, and the pH and temperature were tested respectively. The protein activity was also different at different pH and temperature.

Sodium sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is a method for detecting protein separation effect, which is based on differences in the molecular weight of protein sub-bases. In this experiment, the separation of geland and protein band were studied by SDS-PAGE electrophoresis using SDS-PAGE electrophoresis as a sample of recombinant human follicle-promoting cell hormone (rhFSH). The gel separation effect of different SDS content is different, and the protein bands detected by different sample volumes are different. This experiment first compared the separation effect of 8% and 13.5% of gels, and concluded that the gel with a content of 13.5% was suitable for detecting rhFSH protein. Secondly, change the sample volume on the sample, using the selected 13.5% gel for electrophoresis testing, comparative analysis of the gel map to obtain different sample volume on the gel strip have different effects, suitable for rhFSH sample sample sample volume of 10 sL. At the same time, the protein activity of the sample rhFSH was studied, and the protein activity was different at different pH and temperature, respectively, from pH and temperature.

Twelve sodium alkylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method used to detect protein separation effect. The method is based on the difference of protein subunit molecular weight to achieve protein separation. In this study, recombinant human follicle stimulating hormone (rhFSH) was taken as sample and SDS-PAGE gel electrophoresis was used to study gel separation and protein strip. The gel separation effect of different SDS content is different, and the protein bands detected by different sample volume are different. This experiment first compares the separation effect of 8% and 13.5% gel, and concludes that the gel with 13.5% content is suitable for the detection of rhFSH protein. Secondly, the sample volume was changed, and the 13.5% gel was selected for electrophoresis detection. Compared with the gel graph, the different volume had different effects on the gel strip, which was suitable for rhFSH sample volume of 10 L. At the same time, the protein activity of rhFSH was studied. The protein activity was different at different pH and temperature.<br>