Due to many requests of using the other bodyForschungszentrum Borstel developed, in co-operationand pleural effusion for the T -SPOT`R'.TB assay.fluids then blood for the T-SPOT0. TB thewith Oxford Immunotec, a method of using BALA multicentre study was initiated from theForschungszentrum in Borstel together with the international TB-NET organisation. In this document theseveral procedures should be described.