Populations of Bemisa tabaci MEAM1 were established from nineteen locations in south Florida, primarily from commercial tomato fifields, and were tested using a cotton leaf petiole systemic uptake method for susceptibility to the nicotinic acetylcholine agonist insecticides imidacloprid, thiamethoxam, dinotefuran and flflupyradifurone. Eleven populations produced LC50s for one or more chemicals that were not signifificantly different from the susceptible laboratory colony based on overlapping fifiducial limits, indicating some degree of susceptibility. LC50s more than a 100-fold the laboratory colony were measured in at least one population for each material tested, indicating tolerance. LC50s (ppm) from fifield populations ranged from 0.901–24.952 for imidacloprid, 0.965–24.430 for thiamethoxam, 0.043–3.350 for dinotefuran and 0.011–1.471 for flflupyradifurone. Based on overlapping fifiducial limits, there were no signifificant differences in relative mean potency estimates for flflupyradifurone and dinotefuran in relation to imidacloprid and thiamethoxam.