Fig. 3. Ultrastructure (TEM) of inner leaf tissue treated by vacuum cooling with pressure reduction within 1 h and storage at 1 ◦C and 85%RH for 2 weeks. Bar = 1m (A, B). Abbreviations: m, mitochondria; t, tonoplast; pl, plasmalemma; vl, vesicles; (A) ruptured tonoplastwith vesicles formation as well as organelles moved inward; (B) damaged mitochondrion and unclear tonoplast.