Although the fibre 1 protein of the

Although the fibre 1 protein of the novel FAdV-4 was critical for infection, the cellular receptor of the novel FAdV-4 was unknown. To screen potential receptor candidates, mock LMH cell lysates were mixed with protein A/G sepharose beads that were preincubated with an Fc-tagged anti-fibre 1 antibody. The proteins immunoprecipitated with the anti-fibre 1 antibody were analyzed by LC/MS, which enabled detection of chicken CAR as a ∼38-kilodalton (kD) band visualized by silver staining (Figure 3(A)). Confirmation that chicken CAR was immunoprecipitated with the antifibre 1 antibody was determined by western blot analysis with a chicken CAR-specific antibody (Figure 3(B))