Understanding the factors influenci

Understanding the factors influencing the detection probabilitiescould have significant implications for eDNA survey design and dataanalysis. For example, simple temporal decay conventionally derivedcontributes only fractional to longitudinal eDNA decrease in a river(Nukazawa et al., 2018). eDNA degrades while in transport, either viaabiotic or biotic process such as sedimentation and biofilm, or the inter-action of the two (Shogren et al., 2018). To date, an integrated frame-work that identifies the factors controlling fish eDNA movement andfate in lentic (lakes and ponds) and lotic environments (rivers, streams,and oceans) is lacking (Stewart, 2019).The influencing factors affecting the persistence of eDNA in the fieldand then affecting subsequent monitoring include the following:1) characteristics of eDNA (e.g., molecular conformation, length, corre-lation with cell/organelle membranes) released into thefield; 2) abioticenvironmental factors (e.g., UV-radiation, dissolved oxygen, pH,salinity, water flow, abundance, and composition of the substrate);and 3) biotic factors (e.g., the factors related to the organism itself,biological environmental factors) (Fig. 6).