Due to its easy and quick applicability, Aniline blue was used to visualize callose in various strains of Klebsormidium sp. and Zygnema sp., before more laborious fixation and immunolocalisation protocols were applied (Herburger and Holzinger, 2015). Applying Aniline blue staining and monoclonal antibodies against callose brought similar results (Herburger and Holzinger, 2015). Calcofluor white staining is the fastest way to visualize the 1→4-β-glucan fraction including cellulose of the cell wall, since no pre-treatments are required.