This phenomenon is caused by a higher amount of glucose in the medium as the feed already started after 6 hours. After 23 hours of cultivation, a maximum biomass value of 227.3 a.u. (OD600 = 15.93 ± 0.69) was achieved. During the growth of B. bifidum, lactic acid production occurred and its growth correlated, which is observable in the curve of the addition of NaOH to maintain the pH at pH 6. In total, 193.56 µL of 3 M NaOH were pumped into the culture broth. With a continuous flowrate of 30 mL/min N2, the DO decreased steadily.