绞股蓝，又称：天堂草、福音草、五叶参和七叶参等，葫芦科、绞股蓝属多年生

绞股蓝，又称：天堂草、福音草、五叶参和七叶参等，葫芦科、绞股蓝属多年生草质攀援藤本植物，可以全草入药，其中绞股蓝皂苷为主要的有效成分，具有保肝解毒、降血压、降血脂、降血糖等药理活性。<br>本论文在对绞股蓝中总皂苷含量的提取工艺进行优化的基础上，比较栽培品种和野生品种中绞股蓝总皂苷含量的差异。采用正交试验法优化绞股蓝总皂苷的超声提取条件，以测定样品总皂苷的提取量为考察指标，进行提取工艺的优选，最终确定在乙醇浓度为80%，提取时间60min，提取次数3次，料液比1:40g/ml的条件下为最优提取。再对不同品种的绞股蓝总皂苷含量做比较研究，得栽培品绞股蓝的总皂苷平均含量为7.56%，高于野生品绞股蓝总皂苷含量5.81%，由此可见绞股蓝栽培技术的研究工作己具明显成效，绞股蓝野生资源也仍具有开发潜力。<br>关键词：绞股蓝；总皂苷；正交设计；超声提取

绞股蓝，又称：天堂草、福音草、五叶参和七叶参等，葫芦科、绞股蓝属多年生草质攀援藤本植物，可以全草入药，其中绞股蓝皂苷为主要的有效成分，具有保肝解毒、降血压、降血脂、降血糖等药理活性。<br>本论文在对绞股蓝中总皂苷含量的提取工艺进行优化的基础上，比较栽培品种和野生品种中绞股蓝总皂苷含量的差异。 采用正交试验法优化绞股蓝总皂苷的超声提取条件，以测定样品总皂苷的提取量为考察指标，进行提取工艺的优选，最终确定在乙醇浓度为80%，提取时间60min，提取次数3次，料液比1:40g/ml的条件下为最优提取。 再对不同品种的绞股蓝总皂苷含量做比较研究，得栽培品绞股蓝的总皂苷平均含量为7.56%，高于野生品绞股蓝总皂苷含量5.81%，由此可见绞股蓝栽培技术的研究工作己具明显成效，绞股蓝野生资源也仍具有开发潜力。<br>关键词：绞股蓝；总皂苷；正交设计；超声提取

Gynostemma pentaphyllum, also known as heaven grass, evangelical grass, five leaf ginseng and seven leaf ginseng, etc., is a perennial grass climbing Liana of Cucurbitaceae and gynostemma pentaphyllum, which can be used as medicine for the whole grass. Among them, Gynostemma pentaphyllum saponin is the main effective component, with pharmacological activities such as liver protection, detoxification, blood pressure reduction, blood lipid reduction and blood glucose reduction.<br>In this paper, on the basis of optimizing the extraction process of total saponin content in Gynostemma pentaphyllum, the differences of total saponin content in cultivated and wild varieties were compared. Orthogonal test was used to optimize the ultrasonic extraction conditions of Gypenosides. The extraction amount of total saponins was determined as the index to optimize the extraction process. Finally, the optimal extraction was determined under the conditions of 80% ethanol concentration, 60 minutes of extraction time, 3 times of extraction and 1:40 g / ml of material / liquid ratio. The average content of Gypenosides in cultivated Gynostemma pentaphyllum is 7.56%, which is higher than that in wild Gynostemma Pentaphyllum by 5.81%. It can be seen that the research on Cultivation Technology of Gynostemma pentaphyllum has achieved remarkable results, and the wild resources of Gynostemma pentaphyllum still have development potential.<br>Keywords: Gynostemma pentaphyllum; total saponins; orthogonal design; ultrasonic extraction<br>