. High-performance size-exclusion chromatography was used to check the purity of the samples, particularly to show that SPEs werefree from unreacted fatty acid methyl esters. Thin layer chromatography (TLC), IatroscanTLC flame ionization detection (FID), and high-performance liquid chromatography(HPLC) on reversed phase were applied for the separation of octa-, hepta-, and hexaestersof sucrose. Pure fractions from the total mixture, obtained by column chromatography,were identified by infrared and nuclear magnetic resonance spectroscopy.