A set of extraction, equilibration, and elution buffers optimized for purifying his-tagged proteins using HisTALON Cartridges, TALON Superflow, TALON, and TALON CellThru Resins. The kit is sufficient for sample preparation and up to 20 ÄKTA FPLC automated chromatography runs-and/or purifying up to 400 mg of his-tagged proteins under native (normal operating) conditions.