Experimental ProceduresTransfection

Experimental ProceduresTransfection OptimizationAlthough the transfection protocol below has been shown to result in highly efficient transfection, we recommend that the transfection conditions be optimized for each distinct cell line. The following variables should be considered:A. Cell density (% confluence at transfection): The recommended confluency for most adherent cell types at transfection is 50-70%. The optimal confluency may vary significantly among different cell lines.B. siTran 1.0 to siRNA ratio: We suggest that the conditions should be optimized for each cell line using the conditions in table 1 as a starting point.