After 124 days, at full maturity, t

After 124 days, at full maturity, the plants were harvested and roots, shoots, and grains were separated from each other. At the time of harvesting, the length of shoot and spike was recorded with stainlesssteel meter scale. To remove metals from roots surface, the plants roots were washed by using 0.1 M HCl for two minutes and washed with distilled H2O and all the plant tissues were dried at 70 °C for about 72 h. Thereafter, dry biomasses were noted and all samples were ground to small size and used for the measurement of Cd and Si.