Thermo spore--NQACThermophilic aerobic bacterial spore of NQACStep1. Sample preparation- Prepare 1:10 dilutions in TS broth2. Heat treatmentPipette initial suspension 10 ml to sterile tube 2 tube (control 1 tube) place it in water bath control temp at 100+0.5℃for 30 min.3. Inoculation Pour plate with PCA + starch4. Incubation Incubate in incubator control temp at55℃+1℃ for 48 h+2h5. Reading/interpretation Count the colonies6. Calculation and expression of resultsExpress the results as colony forming units per g or mL.