GSTs能催化谷胱甘肽中巯基基团对不同亲电分子进行亲核攻击,使外源性化

GSTs can catalyze the nucleophilic attack of sulfhydryl groups in glutathione on different electrophilic molecules, so that exogenous compounds can bind to endogenous reduced glutathione (GSH) [144]. The wash-elution procedure separates GST-tagged fusion proteins from cell lysates, so GST is often used to express and purify affinity tags for fusion proteins [145].

GST can catalyze the sulfhydryl groups in glutathione to attack different electrophilic molecules, and make the binding reaction between exogenous compounds and endogenous reduced glutathione (GSH) [144]. Therefore, the fusion protein with GST label can be separated from the cell lysate through the binding washing elution procedure, so GST is often used to express and purify the affinity label of the fusion protein [145].

GSTs can catalyze the nucleophilic attack of sulfhydryl groups in glutathione on different electrophilic molecules, so that exogenous compounds can combine with endogenous reduced glutathione (GSH) [144]. Therefore, the fusion protein with GST tag can be separated from cell lysate by combining-washing-eluting procedure, so GST is often used to express and purify the affinity tag of fusion protein [145].