The morphologies of H. pluvialis cells before and after extraction were detected by SEM and the photographs are shown in Figure 1. As can be seen in Figures 1(a) and 1(b), the untreated cells are dark brown and intact with no signs of pitting or damage to the cell wall. After the treatment of HCl-ACE, the cells become almost white (Figure 1(c)) and the cell wall is damaged and wrinkled strongly (Figure 1(d)), indicating that a majority of astaxanthin in the cells was extracted. The morphologies of the cells treated by HEX- IPA and MET-ACE show similar properties, that is, brown color (Figures 1(e) and 1(g)) and moderate damage of cell wall (Figures 1(f) and 1(h)). The color of the cells treated by oil-soy is almost the same with the untreated cells (Figures 1(a) and 1(i)), and the cell wall is just slightly changed (Figures 1(b) and 1(j)). These results are in agreement with the extraction oil yields and TACs listed in Table 2.