Although in theory this assay can b

尽管理论上该测定法可用于多种<br>细胞类型的多种不同的共培养物中，但是在使用特定细胞类型<br>及其组合时会出现一些限制。即，初级分离株具有有限的复制潜力和寿命<br>，但是可以被永生化细胞绕过。相关地，需要<br>根据细胞类型（短倍加长倍加长倍）和计划<br>的测定长度来仔细控制细胞密度。在共培养生长中配对细胞的主要挑战是找到一种<br>生长培养基，该培养基将为每种细胞类型提供足够的营养以生存，但又<br>不会过度刺激增殖。允许细胞类型相互作用并自我支撑<br>仅凭各种因素的粘附和交换过程就是<br>该模型最令人激动的方面之一，并且在缺乏高血清的情况下，经常导致新的见解，

Although in theory this assay can be used for multiple different co-cultures with a number of <br>cell types, some restrictions arise in the use of particular cell types as well as the <br>combinations thereof. Namely, primary isolates have a limited duplication potential and life <br>span, but this can be bypassed with immortalized cells. Relatedly, cell density needs to be <br>carefully controlled based on the cell type (short vs. long doubling times) and the planned <br>length of the assay. A major challenge in pairing cells in co-culture growth is to find a <br>growth medium that will supply each cell type with enough nutrients to survive, but that also <br>does not overstimulate proliferation. Allowing cell types to interact and support themselves <br>by the mere process of adhesion and exchange of various factors is one of the more exciting <br>aspects of this model and frequently has led to new insight in the absence of the high serum,

虽然理论上这种方法可以用于多种不同的共培养<br>单元格类型，在使用特定单元格类型以及<br>它们的组合。也就是说，原代分离株的复制潜力和寿命有限<br>但这可以通过永生化细胞绕过。相关的，细胞密度需要<br>根据细胞类型（短倍与长倍）和计划的<br>分析的长度。在共培养生长中配对细胞的一个主要挑战是<br>为每一种细胞类型提供足够营养以生存的生长培养基<br>不会过度刺激增殖。允许单元格类型相互作用并支持它们自己<br>仅仅是各种因素的粘附和交换过程，就更令人兴奋了<br>在缺乏高血清的情况下，这一模型的各个方面经常带来新的见解，