针对宁夏灌区盐碱地农田土壤氮素转化微生物过程不清和氮肥利用较低等问题，

In view of the problems of unclear nitrogen conversion microbial process and low nitrogen fertilizer utilization in saline-alkali farmland soils in Ningxia irrigation area, 15N stable isotope, qPCR and other molecular biological methods were used to carry out key microbial processes for nitrogen conversion in saline-alkali farmland soils and nitrogen utilization of exogenous carbon and nitrogen The input response study aims to identify the effects of different farmland management measures such as saline-alkali farmland nutrient management and exogenous carbon / nitrogen input on the microbial process of nitrogen conversion in saline-alkali soils; analyze the composition and distribution characteristics of key microbial communities in nitrogen conversion The relationship between environmental factors; analysis of the contribution and function of microbial functional genes involved in soil nitrogen transformation in the process of nitrogen transformation, clarification of the main controlling factors of nitrogen transformation functional microbial genes in saline and alkaline soils and their response to carbon and nitrogen input in saline and alkaline soils To reveal the microbial driving mechanism of nitrogen transformation in the saline-alkali farmland soil ecosystem, and provide a theoretical basis for optimizing the effective management of nitrogen in saline-alkali farmland and the efficient use of nitrogen fertilizer. Published 4-6 high-quality academic papers through project research, and cultivated a research team with higher research level and innovative ability to improve soil nutrients and microbial regulation in saline soil. <br>Microbial community

In view of the problems such as the unclear process of nitrogen conversion microorganism and the low utilization of nitrogen fertilizer in the farmland of saline-alkali area in Ningxia irrigation area, the molecular biology methods such as 15N stable isotope and qPCR were carried out, the key microbial process of nitrogen conversion in soil soil in salt-alkali land and the response study of nitrogen-based nitrogen input were carried out, aiming to find out the effect son-in-soil nitrogen transformation process of the farmland nutrient management, external carbon/nitrogen input and so on. Analysis of the composition, distribution characteristics of nitrogen transformation key microbial communities and their links with environmental factors, analysis of the contribution of the genes involved in soil nitrogen transformation microbial function in the process of nitrogen transformation and the size of its role, the identification of the main control factors of microbial genes of saline-alkali nitrogen conversion function and its response to the carbon-nitrogen input in saline-alkali soil; Through the project research published 4-6 high-quality academic papers, training a high level of research and innovation of the soil nutrient upgrading and microbial regulation of the research team.<br>Microbial communities

In order to solve the problems of unclear microbial process of nitrogen transformation and low nitrogen utilization in saline alkali soil of Ningxia irrigation area, 15N stable isotope and qPCR were used In order to find out the influence of different management measures, such as nutrient management, carbon / nitrogen input and so on, on the microbial process of nitrogen transformation in saline alkali soil, to analyze the composition, distribution characteristics of the key microbial communities and their relationship with The relationship between environmental factors; the contribution and role of microbial functional genes involved in soil nitrogen transformation in the process of nitrogen transformation; the main control factors of microbial genes involved in soil nitrogen transformation and their response to carbon and nitrogen input in saline alkali soil; the microbial driving mechanism of nitrogen transformation in saline alkali soil agroecosystem was revealed to optimize nitrogen input in saline alkali soil Effective management and efficient utilization of nitrogen provide theoretical basis. Through the project research, 4-6 high-quality academic papers were published, and a research team with high research level and innovation ability for Soil Nutrient Improvement and microbial regulation in saline alkali land was cultivated.<br>Microbial community<br>