DNA profiling based on 16S rRNA phylogeny, electron microscopy and profilometry analyses of the cookies were undertaken. Mass spectrometry characterization of acid washes of the cookies was carried out to identify compounds indicative of microbial metabolism of hydrocarbons. The presence of the biofilm on the surfaces of the cookies was verified through field emission scanning electron microscopy imaging, and both eubacterial and archaeal 16S rRNA sequences were successfully recovered from the biofilms. The presence of metabolites associated with hydrocarbon biodegradation pathways was also confirmed.