Mutate the amino acid about 6 Å away from G protein, and detect the effect of mutation on downstream signal through IP1 experiment. The data are from at least three different experiments (n ≥ 3).
The amino acid which is about 6 miles away from G protein was mutated, and the influence of mutation on downstream signal was detected by IP1 experiment. The data came from at least three different experiments (n≥3).