Figure 3. Discrimination of live and dead cells in fluorescence microscopy using Live-or-Dye™ Fixable Viability Stains. Ethanol-treated HeLa cells were stained with the indicated Live-or-Dye™ cell stain and then stained with Hoechst to label both live and dead cells. Killed cells show bright Live-or-Dye™ fluorescence staining, compared to no staining seen in live cells (blue Hoechst-stained nuclei), allowing the two populations to be clearly distinguished.