中药单体小檗碱，又称黄连素，可以用于心血管系统疾病的治疗，可以作为一种

中药单体小檗碱，又称黄连素，可以用于心血管系统疾病的治疗，可以作为一种心脏保护剂，保护各种心脏毒性。阿霉素（即多柔比星，简称DOX），属于蒽环类抗生素，抗瘤谱广、临床疗效高，是治疗多种恶性肿瘤的一线用药，但由于其具有较高的心肌亲和力，大剂量DOX可导致严重的急性心肌损伤和心脏功能衰竭。DOX 对心脏损伤主要机制是诱导心肌细胞凋亡，DOX所导致的心肌细胞凋亡涉及氧化应激、P53途径、Caspase-3途径、Ca2+途径、P38MARK途径、P13K/Akt途径、DNA 损伤等。小檗碱对DOX所致心肌损伤有明显的对抗作用，但其详细机制尚不清楚。本研究在采用传统动物和细胞实验方法的基础上，又增加了胚胎干细胞衍生的心肌细胞作为研究工具，解决了人源心肌细胞在体外难以获取的难题，可以和动物细胞实验结果比较差异，将从多个角度探讨小檗碱对DOX所致心肌损伤可能的作用机制，以期为小檗碱心脏保护作用的临床应用提供更加确切的理论依据。

中药单体小檗碱，又称黄连素，可以用于心血管系统疾病的治疗，可以作为一种心脏保护剂，保护各种心脏毒性。 阿霉素（即多柔比星，简称DOX），属于蒽环类抗生素，抗瘤谱广、临床疗效高，是治疗多种恶性肿瘤的一线用药，但由于其具有较高的心肌亲和力，大剂量DOX可导致严重的急性心肌损伤和心脏功能衰竭。 DOX 对心脏损伤主要机制是诱导心肌细胞凋亡，DOX所导致的心肌细胞凋亡涉及氧化应激、P53途径、Caspase-3途径、Ca2+途径、P38MARK途径、P13K/Akt途径、DNA 损伤等。 小檗碱对DOX所致心肌损伤有明显的对抗作用，但其详细机制尚不清楚。 本研究在采用传统动物和细胞实验方法的基础上，又增加了胚胎干细胞衍生的心肌细胞作为研究工具，解决了人源心肌细胞在体外难以获取的难题，可以和动物细胞实验结果比较差异，将从多个角度探讨小檗碱对DOX所致心肌损伤可能的作用机制，以期为小檗碱心脏保护作用的临床应用提供更加确切的理论依据。

Berberine, also known as berberine, can be used in the treatment of cardiovascular diseases, and can be used as a cardioprotective agent to protect various cardiac toxicity. Doxorubicin (doxorubicin, DOX) is an anthracycline antibiotic with broad anti-tumor spectrum and high clinical efficacy. Doxorubicin is the first-line drug for the treatment of a variety of malignant tumors. However, due to its high myocardial affinity, high-dose DOX can lead to severe acute myocardial injury and heart failure. The main mechanism of DOX induced cardiac injury is to induce cardiomyocyte apoptosis, which involves oxidative stress, p53 pathway, caspase-3 pathway, Ca2 + pathway, p38mark pathway, P13K / Akt pathway, DNA damage and so on. Berberine has obvious antagonism to dox induced myocardial injury, but its detailed mechanism is still unclear. Based on the traditional animal and cell experimental methods, we added cardiomyocytes derived from embryonic stem cells as a research tool to solve the problem that human cardiomyocytes are difficult to obtain in vitro, which can be compared with the results of animal cell experiments. We will explore the possible mechanism of berberine on DOX induced myocardial injury from multiple perspectives, so as to provide a reference for berberine heart transplantation The clinical application of protective effect provides a more accurate theoretical basis.<br>