HSA-Cre-expressing mice are therefore the most appropriate control littermate when using this Cre-loxP system to generate skeletal muscle-specific gene knockout mice.How might this impact on clinical practice in the foreseeable future?Our findings will lead to the appropriate control littermates being used for studies investigating skeletal muscle-specific regulation of blood glucose in obesity and the metabolic syndrome, possibly leading to higher success rates for translating benchside findings to the bedside.