动物禁食12小时，用戊巴比妥钠（140mg/kg静脉注射）安乐死，立即

The animals were fasted for 12 hours and euthanized with sodium pentobarbital (140 mg/kg intravenous injection). The stomach was immediately resected, cut along a large arc, and fixed with 10% neutral formalin solution. Stomach tissue was perfused with 4% phosphatidic acid 0.1mol/L phosphate buffer, 30% sucrose was cryoprotected in phosphate buffer overnight at 4℃. The specimens were embedded in paraffin, and 4μm specimens were stained with hematoxylin and eosin. According to histological characteristics, the histological results are divided into four types: chronic superficial gastritis, chronic atrophic gastritis, dysplasia and tumor type. The sections were stained with hematoxylin-eosin (H&E) to observe the morphological changes. According to the manufacturer's introduction, the HID-AB-PAS staining method was used to detect the type of intestinal metaplasia, and HID-Alcian blue-periodate Schiff (HID-AB-PAS) staining was performed.

Animals fast for 12 hours, euthanasia with sodium barbiturate (140 mg/kg intravenously), immediately remove the stomach, cut along a large arc, with 10% neutral Folmarin solution fixed. Gastric tissue infusion 4% phospholipidic acid 0.1mol/L phosphate buffer, 30% sucrose in phosphate buffer frozen protection overnight, 4 degrees C. Specimen paraffin enculation buried, 4 m specimen Sumu jing-I red dyeing. Histological results are divided into four types according to histological characteristics: chronic superficial gastritis, chronic atrophy gastritis, heterogeneous growth and tumor type. The slices are dyed with sumu-E to observe morphological changes. According to the manufacturer, the type of enteric chemicals was detected by HID-AB-PAS staining method, and HID-Alcian blue-high iodized acid Schiff (HID-AB-PAS) staining was carried out.

After fasting for 12 hours, the animals were euthanized with pentobarbital sodium (140 mg / kg intravenous injection). The stomach was immediately removed, cut along the great arc, and fixed with 10% neutral formalin solution. Gastric tissue was perfused with 4% phosphatidylcholinic acid 0.1mol/l phosphate buffer, 30% sucrose was frozen in phosphate buffer overnight, 4 ℃. The specimens were embedded in paraffin and stained with hematoxylin eosin at 4 μ M. According to the histological characteristics, the histological results were divided into four types: chronic superficial gastritis, chronic atrophic gastritis, dysplasia and tumor type. Sections were stained with hematoxylin eosin (H & E) to observe the morphological changes. According to the manufacturer's introduction, the type of intestinal metaplasia was detected by hid-ab-pas staining, and hid-alcian blue periodate Schiff (hid-ab-pas) staining was performed.