Bacterial strains, plasmids, and culture conditions.The bacterial strains and plasmids used in this study are listed in Table 1. Escherichia coli and A. baumannii were grown in Luria-Bertani (LB) broth alone or in LB broth containing 1.5% (wt/vol) agar at 37°C. For plasmid maintenance in E. coli, chloramphenicol (20 μg/ml), erythromycin (400 μg/ml), or kanamycin (50 μg/ml) was added to the growth medium. After conjugation, A. baumannii merodiploids were selected on the medium supplemented with ampicillin (100 μg/ml) and kanamycin (30 μg/ml) or erythromycin (30 μg/ml). All the medium components were purchased from Difco (Detroit, MI, USA), and the chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).
細菌菌株、質粒和培養條件。<br>本研究中使用的細菌菌株和質粒列於表1。大腸桿菌和A.鮑曼尼單獨生長在盧里亞-貝爾塔尼(LB)肉湯中,或在37°C下含有1.5%(wt/vol)的LB肉湯中。 對於大腸桿菌的質粒維持,將氯黴素(20微克/毫升)、紅黴素(400微克/毫升)或卡納黴素(50微克/毫升)添加到生長介質中。結合后,在用安西林(100微克/毫升)和卡那黴素(30微克/毫升)或紅黴素(30微克/毫升)補充的介質上選擇A.baumannii merodip體。所有介質元件都是從迪夫科(底特律,美國,美國)購買的,這些化學品是從西格瑪-奧爾德里希(聖路易斯,美國,美國)購買的。
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