石蜡切片放入10%中性甲醛中30分钟，水洗；4℃70%酒精中浸泡1h，

石蜡切片放入10%中性甲醛中30分钟，水洗；4℃70%酒精中浸泡1h，水洗；避光条件下37℃10%硝酸银中浸泡45分钟，水洗；避光条件下在缓冲液内镀银12-24h；取出切片甩掉多余银液，浸入还原液30分钟，水洗；调色后浸入2%草酸水溶液5分钟至神经细胞变色；浸入5%硫代硫酸钠溶液30s，水洗；酒精梯度脱水；二甲苯透明，

石蜡切片放入10%中性甲醛中30分钟，水洗；4℃70%酒精中浸泡1h，水洗；避光条件下37℃10%硝酸银中浸泡45分钟，水洗；避光条件下在缓冲液内镀银12-24h；取出切片甩掉多余银液，浸入还原液30分钟，水洗 ；调色后浸入2%草酸水溶液5分钟至神经细胞变色；浸入5%硫代硫酸钠溶液30s，水洗；酒精梯度脱水；二甲苯透明，

Paraffin section is immersed in 10% neutral formaldehyde for 30 minutes, washed with water; 4 ℃ 70% alcohol for 1 hour, washed with water; 37 ℃ 10% silver nitrate for 45 minutes, washed with water under dark conditions; silver plating in buffer for 12-24 hours under dark conditions; take out the section and shake off the excess silver solution, immerse in reducing solution for 30 minutes, washed with water; after color mixing, immerse in 2% oxalic acid for 5 minutes to change the color of nerve cells; immerse in 5% thiosulfuric acid Sodium solution for 30s, water washing; alcohol gradient dehydration; xylene transparency,<br>