(1) Prepare Golgi solution, dissolve 1g of potassium dichromate in 20ml of double distilled water to make 5% potassium dichromate solution, then dissolve 1g of mercuric chloride in 20ml of double distilled water to make 5% chloride Mercury solution, dissolving 0.8g of potassium chromate in 16ml of double-distilled water to form a 5% potassium chromate solution, and finally mixing 20ml of 5% potassium dichromate solution with 5% mercuric chloride solution, and finally mixing 16ml Potassium chromate solution mixed with 40ml of these solutions evenly, and stored in the dark for more than 5 days before use. (2) Organization preparation. The brains of the mice were removed and placed in the prepared Golgi solution, stored at room temperature in the dark for 14 days, and dehydrated with 75%, 95%, and absolute ethanol in turn. (3) Prepare vibrating slices, hippocampal slices with a thickness of 120 μm, and place them in a 6-well plate. Soak brain slices in 16% ammonia solution for 30 minutes, rinse twice in double distilled water, 2 minutes each time; soak in 1% sodium thiosulfate solution for 7 minutes, rinse twice with double distilled water, 5 minutes each time; Dehydrate in 75%, 95%, and 100% ethanol for 10 min; clear in xylene for 30 s, and seal with neutral gum.
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