Wistar SPF rats were used for a 28

Wistar SPF rats were used for a 28 -day oral toxicity study with Trans-resveratrol. Rats were divided randomly into a negative control group and 3 dose groups (low, medium, and high). The doses for the 4 groups were 0 g/kg BW, 0.25 g/kg BW, 0.75 g/kg BW, and 2.25 g/kg BW, respectively. There were 10 male and 10 female rats in each group. Compared with the negative control group, statistical significance was defined as a p-value of less than 0.05 (p < 0.05). Experimental results are as follows: Compared with the negative control group, the body weight at week 3 and total weight gain (0-4 week) of the female high-dose group decreased and the weight at week 2-4 week and total weight gain (0-4 week) of the male high-dose group decreased. The average food intake of the female high-dose group decreased in the first week, while that of the male high-dose group decreased in the second week. In the female high-dose group, HGB content and LY% decreased, while NEUT% increased; in the male medium and high-dose group, NEUT% increased and LY% decreased. With regard to the biochemical indexes, the content of ALT, TG, and chloridion increased in the female high-dose group, while that of ALT and ALB increased in the male medium-dose group, and that of ALT, TP, ALB, BUN and CHO increased in the male high-dose group, and that of GLU decreased in the male high-dose group. The urine PRO in the male high-dose group was increased, while SG in the male medium and high-dose group was increased. The relative organ weight of the liver and kidney of the female high-dose group and that of the kidney of the male high-dose group were significantly increased. There were no toxicological changes related to the test subjects in the daily observation, body weight, food intake, food utilization rate, hematological and biochemical examination, urine examination, organ weighing, organ coefficient and histopathological examination of rats in the other dose groups.Conclusion: In the present experiment, the No Observed Adverse Effect Level (NOAEL) of the 28 days oral toxicity test of Trans-resveratrol was set to be 0.75 g/kg BW in female rats and 0.25g/kg BW in male rats, respectively. The Lowest Observed Adverse Effect Level (LOAEL) was 2.25g/kg BW in females and 0.75 g/kg BW in males, respectively.