Cell invasion assayTranswell invasion assay was performed to assess ce的简体中文翻译

Cell invasion assayTranswell invasi

Cell invasion assayTranswell invasion assay was performed to assess cell invasion. The upper surface of the filter (pore size, 8.0 μm;Biosciences, Heidelberg, Germany) was coated with basement membrane Matrigel (BD Bioscience) at a concentrationof 2 mg/ml and incubated at 4◦C for 3 h. Cells (2 × 104) were seeded into the upper chamber with 200 μl serum-freemedium. The lower chamber was supplemented with 750 μl medium containing 10% FBS. Following incubation for24 h at 37◦C, cells were fixed with 4% polyoxymethylene and stained with 0.5% crystal violet (Sigma–Aldrich). Then,stained cells were counted under a microscope. Five visual fields were selected and the average number was calculated.
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结果 (简体中文) 1: [复制]
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细胞侵袭测定进行<br>Transwell侵袭测定以评估细胞侵袭。滤膜的上表面(孔径为8.0μm;<br>Biosciences,海德堡,德国)<br>以2 mg / ml 的浓度涂有基底膜基质胶(BD Bioscience),并在4℃下孵育3 h。将细胞(2×104)接种到装有200μl无血清<br>培养基的上室中。在下腔室中添加750μl含10%FBS的培养基。<br>在37 ℃下孵育24小时后,将细胞用4%聚甲醛固定,并用0.5%结晶紫(Sigma–Aldrich)染色。然后,<br>在显微镜下计数染色的细胞。选择五个视野并计算平均值。
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结果 (简体中文) 2:[复制]
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Cell invasion assay<br>Transwell invasion assay was performed to assess cell invasion. The upper surface of the filter (pore size, 8.0 μm;<br>Biosciences, Heidelberg, Germany) was coated with basement membrane Matrigel (BD Bioscience) at a concentration<br>of 2 mg/ml and incubated at 4◦C for 3 h. Cells (2 × 104) were seeded into the upper chamber with 200 μl serum-free<br>medium. The lower chamber was supplemented with 750 μl medium containing 10% FBS. Following incubation for<br>24 h at 37◦C, cells were fixed with 4% polyoxymethylene and stained with 0.5% crystal violet (Sigma–Aldrich). Then,<br>stained cells were counted under a microscope. Five visual fields were selected and the average number was calculated.
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结果 (简体中文) 3:[复制]
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细胞侵袭试验<br>采用跨孔侵袭试验评估细胞侵袭性。过滤器的上表面(孔径,8.0μm;<br>生物科学,海德堡,德国)以一定浓度涂有基底膜基质凝胶(BD Bioscience)<br>将细胞(2×104)接种于200μl无血清的上腔中<br>中等。下室补充750μl培养基,含10%FBS。培养后<br>在37℃下24小时,用4%聚甲醛固定细胞,用0.5%结晶紫(Sigma-Aldrich)染色。然后,<br>染色细胞在显微镜下计数。选取5个视野,计算平均视野数。<br>
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