ABSTRACT: Objective: to explore the method of preoperative iodoformol angiography and laparoscopic minimally invasive resection of the first branchial Fistula. Methods: The diagnosis of the first branchial Fistula was confirmed by IODOFORMOL angiography before operation, and the course of the Fistula was judged. Results: Strict follow-up after operation, the prognosis was good, no recurrence of Fistula. Conclusion: IFORMOL angiography can not only make clear the diagnosis of the first branchial Fistula, but also make sure the course of the FISTULA and guide the operation, it is an effective method to resect the first branchial Fistula by laparoscope before operation.