Human fibrinogen polymerized into a

通过与凝血酶的酶促反应聚合成多孔凝胶的人纤维蛋白原被配制为封装功能性和有活力的 T 细胞，这些 T 细胞可以在肿瘤切除腔内原位形成凝胶后局部释放（图 1A）。为了评估分布在整个纤维蛋白凝胶网络中的孔是否容纳 T 细胞的负载，通过将含有纤维蛋白原的溶液与 T 细胞和含有凝血酶的溶液混合，快速形成纤维蛋白凝胶。使用冷冻扫描电子显微镜 (cryo-SEM) 成像证实了 T 细胞在交联纤维蛋白凝胶的孔中的截留（图 1B）。此外，在聚合时，共聚焦显微镜显示封装的 T 细胞均匀分布在荧光素标记的纤维蛋白原内（图 1C）。

Human fibrinogen polymerized into a porous gel by enzymatic reaction with thrombin was formulated to encapsulate functional and viable T cells that can be released locally after the gel was formed in situ in the tumor resection cavity (Fig. 1A). To evaluate whether the pores distributed throughout the fibrin gel network accommodate the loading of T cells, fibrin gel was quickly formed by mixing the solution containing fibrinogen with T cells and the solution containing thrombin. The entrapment of the T cells in the pores of the cross-linked fibrin gel was confirmed using cryo–scanning electron microscopy (cryo-SEM) imaging (Fig. 1B). In addition, upon polymerization, confocal microscopy revealed that encapsulated T cells were uniformly distributed within the fluorescein-labeled fibrinogen (Fig. 1C).<br>

通过与凝血酶的酶促反应聚合成多孔凝胶的人纤维蛋白原被配制成封装功能性和存活的T细胞，该T细胞可在凝胶在肿瘤切除腔中原位形成后局部释放(图1A)。为了评估分布在整个纤维蛋白凝胶网络中的孔是否适应T细胞的负载，通过将含有纤维蛋白原的溶液与T细胞和含有凝血酶的溶液混合来快速形成纤维蛋白凝胶。使用冷冻扫描电子显微镜(冷冻扫描电子显微镜)成像证实了T细胞在交联纤维蛋白凝胶的孔中的截留(图1B)。此外，聚合后，共聚焦显微镜显示包裹的T细胞均匀分布在荧光素标记的纤维蛋白原中(图1C)。